Journal: bioRxiv

Article Title: Identification of a neural basis for anorexia nervosa

doi: 10.64898/2026.02.07.704578

Figure Lengend Snippet: (A) Representative images showing GFP and c-Fos (arrows) expression patterns in the PVT, a downstream target of the VMH, in AAV-DIO-EYFP, AAV-DIO-NaChBac+sgRNA, AAV-DIO-NaChBac+sgVglut2 injection SF1-Cre::ERa-Cre mice. scale bar=250 um. (B) Representative images showing GFP (green) and c-Fos (red, arrows) expression patterns in the PVT, a downstream target of the VMH, in SF1-Cre::ERa-Cre::BDNFflox/++EYFP, SF1-Cre::ERa-Cre::BDNFflox/++NaChBac, SF1-Cre::ERa-Cre::BDNFflox/flox+NaChBac mice. scale bar=250 um. (C) Number of c-Fos positive cells in the PVT region related to panel A. n=4-5 per group; one-way ANOVA; ***P<0.001 versus EYFP; ###P<0.001 versus NaChBac+sgRNA. (D) number of c-Fos positive cells in the PVT region related to panel A. n=4-5 per group; one-way ANOVA; ***P<0.001 versus EYFP; ###P<0.001 versus SF1-Cre::ERa-Cre:: BDNFflox/flox+NaChBac. (E) Diagram of viral delivery (AAV-DIO-mCherry, AAV-DIO-BDNF-mCherry+sgRNA, AAV-DIO-BDNF-mCherry+sgVglut2) into the VMH of SF1-Cre::ERa-Cre mice. (F) Body weight trajectories of SF1-Cre::ERa-Cre mice after VH injection of virus. n=4 (EYFP, BDNF+sgVglut2), n=5 (BDNF+sgRNA); two-way ANOVA; *P<0.05, **P<0.01, ***P<0.001 versus mCherry; #P<0.05 versus BDNF+sgRNA. (G) Daily food intake. n=4-5 per group, one-way ANOVA, **P=0.006 versus mCherry; #P=0.043 versus BDNF+sgRNA. (H) Total number of jumps during a 10-min test. n=4-5 per group; one-way ANOVA; ***P<0.01 versus mCherry; ###P<0.001 versus BDNF+sgRNA. (I, J) time spent in the light room in light-dark test (I), social time with stranger in three-chamber test (J). n=4-5 per group; one-way ANOVA; *P<0.05, ***P<0.01 versus mCherry; #P<0.05 versus BDNF+sgRNA. (K) Representative images showing mCherry (green) and c-Fos (magenta, arrows) expression patterns in the PVT, a downstream of VMH, in mCherry, BDNF+sgRNA, BDNF+sgVglut2 injection SF1-Cre::ERa-Cre mice. scale bar=250 um. (L) number of c-Fos positive cells in PVT region related to panel K. n=4-5 per group; one-way ANOVA; ***P<0.001 versus mCherry; ###P<0.001 versus BDNF+sgRNA.

Article Snippet: The following primary antibodies were used: rabbit anti-cFos (1:1000, #2250, Cell Signaling Technology), rabbit anti-BDNF(1:200, ANT-010, Alomone Labs), goat anti-GFP (1:1000, 51370, ROCKLAND), rabbit anti-ER alpha (1:500, NBP1-84827), chicken anti-mCherry (1:500, MCHERRY-0020, aves labs).The next day, sections were rinsed three times in PBS with 0.1% Triton X-100 and incubated with secondary antibodies prepared in the same diluent (secondary antibody, 5% normal donkey serum, 0.1% Triton X-100 in PBS).

Techniques: Expressing, Injection, Virus

Journal: bioRxiv

Article Title: Identification of a neural basis for anorexia nervosa

doi: 10.64898/2026.02.07.704578

Figure Lengend Snippet: Representative images showing mCherry (green) and c-Fos (magenta, arrows) expression pattern in lateral septum (A), preoptic area (C), PAG (E), periLC (G). scale bar=250 μm. The pictures in the bottom row of each panel represent the magnified pictures shown in the boxed area in each column. Number of cFos positive cells in in lateral septum (B), preoptic area (D), PAG (F, periLC (H). n=4-5 per group; one-way ANOVA; ***P<0.001 versus mCherry; ###P<0.001 versus BDNF+sgRNA.

Article Snippet: The following primary antibodies were used: rabbit anti-cFos (1:1000, #2250, Cell Signaling Technology), rabbit anti-BDNF(1:200, ANT-010, Alomone Labs), goat anti-GFP (1:1000, 51370, ROCKLAND), rabbit anti-ER alpha (1:500, NBP1-84827), chicken anti-mCherry (1:500, MCHERRY-0020, aves labs).The next day, sections were rinsed three times in PBS with 0.1% Triton X-100 and incubated with secondary antibodies prepared in the same diluent (secondary antibody, 5% normal donkey serum, 0.1% Triton X-100 in PBS).

Techniques: Expressing

Journal: bioRxiv

Article Title: Identification of a neural basis for anorexia nervosa

doi: 10.64898/2026.02.07.704578

Figure Lengend Snippet: Representative images showing mCherry (red) and cFos (cyan, arrows) expression pattern in VH in mCherry, BDNF+sgRNA, BDNF+sgVglut2 injection SF1-Cre::ERa-Cre mice (related to ). Scale bar=200 μm.

Article Snippet: The following primary antibodies were used: rabbit anti-cFos (1:1000, #2250, Cell Signaling Technology), rabbit anti-BDNF(1:200, ANT-010, Alomone Labs), goat anti-GFP (1:1000, 51370, ROCKLAND), rabbit anti-ER alpha (1:500, NBP1-84827), chicken anti-mCherry (1:500, MCHERRY-0020, aves labs).The next day, sections were rinsed three times in PBS with 0.1% Triton X-100 and incubated with secondary antibodies prepared in the same diluent (secondary antibody, 5% normal donkey serum, 0.1% Triton X-100 in PBS).

Techniques: Expressing, Injection

Journal: Journal of Perinatal Medicine

Article Title: Nicotine affects the expression of brain-derived neurotrophic factor mRNA and protein in the hippocampus of hypoxic newborn piglets

doi: 10.1515/jpm.2009.081

Figure Lengend Snippet: Figure 1 Relative expression of BDNF mRNA in hippocampus. The group treated with nicotine 130 mg/kg/h show a significant increase in BDNF mRNA expression compared to the group treated with saline. 1snicotine 130 mg/kg/h; 2snicotine 260 mg/kg/h; 3sadrena- line 0.05 mg/kg/minqsaline; 4ssaline. *Ps0.038 for nicotine 130 mg/kg/h vs. saline.

Article Snippet: Monoclonal anti-human BDNF antibody titer 1:25 (Clone 35928; R&D Systems, Minneapolis, MN, USA) was used as primary antibody.

Techniques: Expressing, Saline

Journal: Journal of Perinatal Medicine

Article Title: Nicotine affects the expression of brain-derived neurotrophic factor mRNA and protein in the hippocampus of hypoxic newborn piglets

doi: 10.1515/jpm.2009.081

Figure Lengend Snippet: Figure 4 Immunohistochemistry of BDNF protein, nicotine vs. saline. Pictures from the dentate gyrus 20=enlarged. A is from an ani- mal treated with nicotine 130 mg/kg/h; B is from an animal treat- ed with saline; picture A presents a larger number of BDNF positive cells than picture B.

Article Snippet: Monoclonal anti-human BDNF antibody titer 1:25 (Clone 35928; R&D Systems, Minneapolis, MN, USA) was used as primary antibody.

Techniques: Immunohistochemistry, Saline

Journal: Journal of Perinatal Medicine

Article Title: Nicotine affects the expression of brain-derived neurotrophic factor mRNA and protein in the hippocampus of hypoxic newborn piglets

doi: 10.1515/jpm.2009.081

Figure Lengend Snippet: Figure 2 Box plot of % BDNF positive cells in the dentate gyrus of the hippocampus. There are significantly more BDNF positive cells in the dentate gyrus of the animals treated with nicotine 130 mg/kg/h com- pared to the saline treated animals. *Ps0.009.

Article Snippet: Monoclonal anti-human BDNF antibody titer 1:25 (Clone 35928; R&D Systems, Minneapolis, MN, USA) was used as primary antibody.

Techniques: Saline

Journal: Journal of Perinatal Medicine

Article Title: Nicotine affects the expression of brain-derived neurotrophic factor mRNA and protein in the hippocampus of hypoxic newborn piglets

doi: 10.1515/jpm.2009.081

Figure Lengend Snippet: Figure 3 Immunohistochemistry of BDNF protein. Picture from the dentate gyrus 40=enlarged. The cells with the large, dark brown nucleuses are positive (black arrows), the cells with light brown or blue nucleuses are negative (open arrows).

Article Snippet: Monoclonal anti-human BDNF antibody titer 1:25 (Clone 35928; R&D Systems, Minneapolis, MN, USA) was used as primary antibody.

Techniques: Immunohistochemistry

Journal: Neurochemistry international

Article Title: Water treadmill training protects the integrity of the blood-spinal cord barrier following SCI via the BDNF/TrkB-CREB signalling pathway.

doi: 10.1016/j.neuint.2020.104945

Figure Lengend Snippet: Fig. 1. TT upregulated the BDNF/TrkB-CREB signalling pathway and prevented the loss of TJ and AJ proteins following SCI. (A–H) Representative western blots and quantification data for BDNF/β-Actin, PI3K/β-Actin, p-TrkB/TrkB, p-AKT/AKT and p-CREB/CREB in each group, columns represent the mean ± SD, n = 5. (I) Double staining for Claudin-5, Occludin, p120-Catenin, and β-Catenin/CD31/Hoechst. Red: Claudin-5/Occludin/p120-Catenin/β-Catenin; green: CD31; blue: Hoechst. Scale bar, 20 μm #p < 0.05 for the M group versus the S group, *p < 0.05 for the TM group versus the M group. (#p, *p < 0.05; ##p, **p < 0.01; ###p, ***p < 0.001).

Article Snippet: Anti-BDNF, anti-TrkB and anti-p-TrkB antibodies were purchased from Proteintech (Rosemont, IL, USA). β-Actin was purchased from Abcam (330 Cambridge Science Park, Cambridge, UK).

Techniques: Western Blot, Double Staining

Journal: Neurochemistry international

Article Title: Water treadmill training protects the integrity of the blood-spinal cord barrier following SCI via the BDNF/TrkB-CREB signalling pathway.

doi: 10.1016/j.neuint.2020.104945

Figure Lengend Snippet: Fig. 2. The inhibitor downregulated the BDNF/TrkB-CREB signalling pathway and increased the permeability of the spinal cord, accompanied by poor motor function recovery. (A–H) Representative western blots and quantification data of BDNF/β-Actin, PI3K/β-Actin, p-TrkB/TrkB, p-AKT/AKT and p-CREB/CREB in the M and IM groups. Columns represent the mean ± SD, n = 5. (I) BBB scores in M, IM groups. (J, L) Representative quantification data of spinal cord water content in the M and IM groups; columns represent the mean ± SD, n = 5. (K) Representative Evans blue dye permeabilized into the spinal cord after SCI and (M) quantification of the amount of Evans blue (μg/g), n = 5. $p < 0.05 for the IM group versus the M group ($p < 0.05; $$p < 0.01; $$$p < 0.001).

Article Snippet: Anti-BDNF, anti-TrkB and anti-p-TrkB antibodies were purchased from Proteintech (Rosemont, IL, USA). β-Actin was purchased from Abcam (330 Cambridge Science Park, Cambridge, UK).

Techniques: Permeability, Western Blot

Journal: Neurochemistry international

Article Title: Water treadmill training protects the integrity of the blood-spinal cord barrier following SCI via the BDNF/TrkB-CREB signalling pathway.

doi: 10.1016/j.neuint.2020.104945

Figure Lengend Snippet: Fig. 5. TT decreased endothelial permeability and improved functional recovery by upregulating the BDNF/TrkB-CREB signalling pathway. (A, C) Representative quantification data of spinal cord water content in the TM and ITM groups. Columns represent the mean ± SD, n = 5. &p < 0.05 for the ITM group versus the TM group (B, D) Representative Evans blue dye permeabilized into the spinal cord after SCI and quantification of the amount of Evans blue (μg/g), n = 5. (E) BBB scores in TM and ITM groups. (F) TEM shows the vascular EC-EC junctions in the TM and ITM. Arrows indicate an open tight junction and the scale bars are 0.5 μm. (G) Quantification of the TJ gap width between the TM and ITM; columns represent the mean ± SD. (H) Quantification of the TJ length between the TM and ITM; columns represent the mean ± SD. (&p < 0.05; &&p < 0.01; &&&p < 0.001).

Article Snippet: Anti-BDNF, anti-TrkB and anti-p-TrkB antibodies were purchased from Proteintech (Rosemont, IL, USA). β-Actin was purchased from Abcam (330 Cambridge Science Park, Cambridge, UK).

Techniques: Permeability, Functional Assay

Journal: Neurochemistry international

Article Title: Water treadmill training protects the integrity of the blood-spinal cord barrier following SCI via the BDNF/TrkB-CREB signalling pathway.

doi: 10.1016/j.neuint.2020.104945

Figure Lengend Snippet: Fig. 6. TT inhibited BSCB disruption by upregulating the BDNF/TrkB-CREB signalling pathway. (A, B) Representative western blots and quantification data of TJ and AJ proteins in the TM and ITM groups. Columns represent the mean ± SD, n = 5. (C) Claudin- 5, Occludin, p120-Catenin, and β-Catenin/CD31/ Hoechst staining of sections from the spinal cord in the TM and ITM groups. Red: Claudin-5/Occludin/ p120-Catenin/β-Catenin; green: CD31; blue: Hoechst. Scale bar, 20 μm. (&p < 0.05; &&p < 0.01; &&&p < 0.001).

Article Snippet: Anti-BDNF, anti-TrkB and anti-p-TrkB antibodies were purchased from Proteintech (Rosemont, IL, USA). β-Actin was purchased from Abcam (330 Cambridge Science Park, Cambridge, UK).

Techniques: Disruption, Western Blot, Staining

Journal: Journal of Anesthesia

Article Title: Effects of sevoflurane exposure during different stages of pregnancy on the brain development of rat offspring

doi: 10.1007/s00540-021-02972-2

Figure Lengend Snippet: Effect of gestational sevoflurane anesthesia on BDNF and CPEB2 in the hippocampi of offspring rats. A BDNF expression was detected by immunohistochemistry; B CPEB2 expression was detected by immunohistochemistry. The magnification is 40 × in the first set of images and 200 × in the second set. The red arrow represents positive expression. There were 6 rats in each group, and the data are expressed as \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\overline{X}$$\end{document} X ¯ ± SD

Article Snippet: Subsequently, they were blocked with bovine serum albumin (5% BSA, A8010, Solarbio, Beijing, China) for 20 min. Polyclonal primary antibodies, comprising rabbit anti-human BDNF antibody (1:300, orb10181, Biorbyt, Wuhan, China) and rabbit anti-human CPEB2 antibody (1:300, orb182706, Biorbyt, Wuhan, China), were added and reacted for one night at 4 °C.

Techniques: Expressing, Immunohistochemistry